clinical case origin host geographical location reference 16s rrna sequencing multiplexpcr maldi tof ms species identity (ATCC)

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ATCC clinical case origin host geographical location reference 16s rrna sequencing multiplexpcr maldi tof ms species identity

Figure 1. Phylogenetic tree based on <t>16S</t> <t>rRNA</t> gene sequences showing the phylogenetic positions of Brazilian isolates (in black) among the four Flavobacterium species reported by LaFrentz et al. [10] (F. oreochromis in red, F. columnare in blue, F. covae in green, and F. davisii in yellow) and other Flavobac- teriaceae isolates (in purple). Relatedness was inferred using the maximum likelihood method based upon the General Time-Reversible model [GTR + G + I; 50] and rooted with Capnocytophaga ochracea ATCC 27872T. The percentage of trees in which the associated sequences clustered together in the bootstrap test (1000 replicates) is shown next to the main branches. The analysis involved 58 nucleotide sequences, all positions containing gaps and missing data were eliminated, and there was a total of 1255 positions in the final dataset.

Clinical Case Origin Host Geographical Location Reference 16s Rrna Sequencing Multiplexpcr Maldi Tof Ms Species Identity, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 6380 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Identification and Phylogenetic Analysis of Flavobacterium spp. Associated with Aquaculture Fish Diseased from Brazil."

Article Title: Identification and Phylogenetic Analysis of Flavobacterium spp. Associated with Aquaculture Fish Diseased from Brazil.

Journal: Pathogens (Basel, Switzerland)

doi: 10.3390/pathogens14030219

Figure 1. Phylogenetic tree based on 16S rRNA gene sequences showing the phylogenetic positions of Brazilian isolates (in black) among the four Flavobacterium species reported by LaFrentz et al. [10] (F. oreochromis in red, F. columnare in blue, F. covae in green, and F. davisii in yellow) and other Flavobac- teriaceae isolates (in purple). Relatedness was inferred using the maximum likelihood method based upon the General Time-Reversible model [GTR + G + I; 50] and rooted with Capnocytophaga ochracea ATCC 27872T. The percentage of trees in which the associated sequences clustered together in the bootstrap test (1000 replicates) is shown next to the main branches. The analysis involved 58 nucleotide sequences, all positions containing gaps and missing data were eliminated, and there was a total of 1255 positions in the final dataset.

Figure Legend Snippet: Figure 1. Phylogenetic tree based on 16S rRNA gene sequences showing the phylogenetic positions of Brazilian isolates (in black) among the four Flavobacterium species reported by LaFrentz et al. [10] (F. oreochromis in red, F. columnare in blue, F. covae in green, and F. davisii in yellow) and other Flavobac- teriaceae isolates (in purple). Relatedness was inferred using the maximum likelihood method based upon the General Time-Reversible model [GTR + G + I; 50] and rooted with Capnocytophaga ochracea ATCC 27872T. The percentage of trees in which the associated sequences clustered together in the bootstrap test (1000 replicates) is shown next to the main branches. The analysis involved 58 nucleotide sequences, all positions containing gaps and missing data were eliminated, and there was a total of 1255 positions in the final dataset.

Techniques Used:

Luciferase:

Article Title: Construction and Application of Orthogonal T7 Expression System in Eukaryote: An Overview.
Article Snippet: DOI: 10.1002/adbi.202200218 to its high efficiency and yield for protein synthesis.. [12] Among the commonlyused protein expression systems in E. coli (Table 1), T7 expession system prepared more than 90% of proteins in protein data bank (PDB) due to its high yield and translation efficiency for target protein.. [13] The T7 system had been applied extensively for target protein and mRNA synthesis in prokaryote, eukaryote, and cell free system.

Sequencing:

Article Title: Identification and Phylogenetic Analysis of Flavobacterium spp. Associated with Aquaculture Fish Diseased from Brazil.
Article Snippet: .. \ Clinical Case Origin Host Geographical Location Reference 16S rRNA Sequencing MultiplexPCR MALDI-ToF MS Species Identity (%) Coverage (%) Species Before Custom MSP Inclusion † Organism Best Match After Custom MSP Inclusion Score Value † Score Value ATCC 23463 - USA Oncorynchus tshawytscha [28] F. columnare 99.93 f 100 F. columnare 1.441 F. columnare 2.057 ATCC 49512 - France Salmo truta [29] F. columnare 99.57 g 100 F. columnare 1.562 F. columnare 2.601 Lowercase letters indicate strains used for NCBI identities and their accession numbers: a TI2056 (KX711900.1); b AL-02-36 (MW353013.1); c C#2 (CP015107.1); d CUVET1215 (KF274040.1); e TI1690 (KX711899.1); f B185R (CP013012.1); g 2023717G (OR946449.1). ..

Article Title: First report of whole genome sequence of septicemic Pasteurella multocida serovar B:2 'Soron' strain isolated from swine.
Article Snippet: Pig pasteurellosis, caused by Pasteurella multocida, is an acute infection that also has economic implications for pig farmers.. We report the complete genome sequence of a P. multocida, serovar B:2 ‘Soron’ strain isolated from the blood of a pig that had died of pasteurellosis in India.. The isolate was not found to be haemorrhagic septicaemia (HS) specific B:2 by the PCR assay.

Transformation Assay:

Article Title: Method and composition for inhibiting growth of bacterium
Article Snippet: .. TABLE 1 Source or P. aeruginosa strain Description reference PA51530 (S1) Clinical isolate, Sensitive Laboratory collection PA83365 (S2) Clinical isolate, Sensitive Laboratory collection PA85710 (S3) Clinical isolate, Sensitive Laboratory collection PA191712 (R1) Clinical isolate, Multidrug Laboratory resistance collection PA194803 (R2) Clinical isolate, Multidrug Laboratory resistance collection PA185388 (R3) Clinical isolate, Mutidrug Laboratory resistance collection R3-AS1974 R3 transformed with This Study pMMB66EH-rrnBter-tet- AS1974 R3-AS1974-Reverse R3 transformed with This Study pMMB66EH-rrnBter-tet- AS1974-Reverse R3-IGR2780 R3 transformed with This Study pMMB66EH-rrnBter-tet- IGR2780 R3-AS2779 R3 transformed with This Study pMMB66EH-rrnBter-tet- AS2779 R3-Empty Vector R3 transformed with This Study pMMB66EH-rrnBter-tet Source or Plasmid(s) Description reference pMMB66EH Board-host-range expression ATCC vector; Apr pMMB66EH-tet Insert Transcription Unit of This Study tet via Pst I site, Apr Tetr pMMB66EH-rrnBter-tet Insert xba I-xho I-kpn I-rrnB This Study terminator via Eco RI site, Apr Tetr pMMB66EH-rrnBter-tet- 0.128-kb xho I-kpn I AS1974 This Study AS1974 fragment cloned into the same site of pMMB66EH-rrnBter-tet pMMB66EH-rrnBter-tet- 0.128-kb xho I-kpn I reverse This Study AS1974-Reverse complement of AS1974 fragment cloned into the same site of pMMB66EH- rrnBter-tet pMMB66EH-rrnBter-tet- 0.059-kb xho I-kpn I IGR2780 This Study IGR2780 fragment cloned into the same site of pMMB66EH-rrnBter-tet pMMB66EH-rrnBter-tet- 0.129-kb xho I-kpn I AS2779 This Study AS2779 fragment cloned into the same site of pMMB66EH-rrnBter-tet .. The MICs of 11 antibiotics, including penicillin G, cefotaxime, chloramphenicol, tetracycline, erythromycin, lincomycin, ciprofloxacin (Sigma, St. Louis, Mo., USA), levofloxacin (Daiichi Sankyo, Tokyo, Japan), gatifloxacin (Bristol-Myers Squibb, Moreton, UK), moxifloxacin (Bayer Healthcare Pharmaceuticals, Berlin, Germany), and linezolid (Pfizer, NJ, USA), were determined using the microbroth dilution method, according to the Clinical and Laboratory Standards Institute (CLSI).

Plasmid Preparation:

Article Title: Three separate pathways in Rhizobium leguminosarum maintain phosphatidylcholine biosynthesis, which is required for symbiotic nitrogen fixation with clover
Article Snippet: .. Strain or plasmid Relevant characteristics Source or reference Strain Escherichia coli JM83 Cloning host (1) E. coli BL21 (DE3) Host for gene expression (2) E. coli S17-1 Donor for biparental mating (3) Rhizobium leguminosarum bv. trifolii ATCC 14479 (also DSM 6040; 3D1K2) Wildtype DSMZ-German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany R. leguminosarum ΔpmtS1 Wildtype derivative, deletion of the pmtS1 gene This study R. leguminosarum ΔpmtS2 Wildtype derivative, deletion of the pmtS2 gene This study R. leguminosarum ΔpmtS3 Wildtype derivative, deletion of the pmtS3 gene This study R. leguminosarum ΔpmtR1 Wildtype derivative, deletion of the pmtR1 gene This study R. leguminosarum Δpcs Wildtype derivative, deletion of the pcs gene This study ..

Expressing:

Clone Assay:

Cloning:

Gene Expression:

Incubation:

Article Title: Effect of the Biopolymer Carrier on Staphylococcus aureus Bacteriophage Lytic Activity.
Article Snippet: .. A solid agar plate was inoculated with the host strain (reference strain S. aureus (ATCC 25923)) and incubated overnight at 37 ◦C. .. Furthermore, the 3–5 bacterial colonies grown on the plate were inoculated in a liquid medium (trypticase soy broth (TSB, Oxoid, Basingstoke, Hampshire, UK)) and incubated overnight at 37 ± 1 ◦C.

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